Буссо Дж.М. и др. Неинвазивный мониторинг эндокринной активности яичников шиншиллы (англ.)
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Abstract
Reproductive endocrinology information is limited for Chinchilla lanigera, a South American species characterized by extremely long gestation and estrus cycle compared with others rodents. This study was designed to validate a non-invasive technique for monitoring ovarian endocrine activity. Animals were exposed indoors to natural photoperiod (31°S–64°W, Argentina); temperature range: 17–26°C, with food and water ad libitum. Radiolabelled infusion (n=4): 3H-estradiol ( 3H-E2 ) and 14C-progesterone ( 14C-P4 ) were injected (i.p). Biochemical validation: HPLC-UV detector was employed to determine natural steroids in urine and fecal extracts and to determine immunoreactive metabolites. Physiological validation: (1) pregnancy (n=5): body weight and urinary and fecal steroidal metabolites were measured until birth; (2) Seasonality (n=9): urine and feces were collected in May, August, November, and February. Total 3H-E2 and 14C-P4 radioactivity recovered was 60.5÷15.5 and 74.5÷19.4%, respectively. After 3H-E2 injection, urinary radioactivity peaked at 7.0÷0.6hr; in contrast, urinary 14C-P4 excretion peaked at 44.0÷4.0 hr (p=0.000). Peak radioactivity in feces occurred between 24–48 hr for both hormones. Several correlations were detected during pregnancy between body weight vs. urinary progestagens/day (r=0.44, p<0.03); vs. urinary progestagens/creatinine (r=0.73, p=2.9x10-5 ); vs. urinary estrogens/day (r=0.74; p<0.2); and vs. urinary estrogens/creatinine (r=0.74; p<2.0x10-5 ). On the other hand, urinary and fecal progestagen excretion exhibited significant seasonal fuctuations and urinary estrogen concentrations showed a similar pattern (p=0.062 for winter–spring vs. summer–autumn). This methodology proved to be useful for monitoring ovary endocrine activity in urine of chinchilla female.
Busso J.M., Ponzio M.F., de Cuneo M.F., Ruiz R.D. Noninvasive monitoring of ovarian endocrine activity in the chinchilla (Chinchilla lanigera) (Неинвазивный мониторинг эндокринной активности яичников шиншиллы) // General and Comparative Endocrinology. - 2007. - N150. - Pp. 288–297.
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